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10.17875/gup2016-953

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Characterisation of the yeast RNA helicase Prp43

von Annika Heininger
Erschienen: 2016
 
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Softcover, 17x24; 88 Seiten
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RNA helicases have many important roles in cell metabolism and are involved in numerous pathways, e.g. translation, pre-mRNA splicing and biogenesis. Interestingly, an increasing number of RNA helicases have been shown to be required for more than one cellular pathway. Therefore, protein cofactors have been suggested to recruit multifunctional RNA helicases to their substrates and regulate their activity. The best characterised family of helicase cofactors are G-patch proteins, which all contain a glycine-riche domain. This work focuses on the characterisation of the yeast RNA helicase Prp43 and its G-patch protein cofactors. Additionally, a new G-patch protein cofactor of Prp43 was identified and characterised. Together, the data suggest that protein cofactors can regulate the distribution and activity of RNA helicases in different pathways.

  • INDEX
  • SUMMARY
  • 1. INTRODUCTION
    • 1.1. RNA helicases
    • 1.2. Pre-mRNA splicing
    • 1.3. Ribosome biogenesis
    • 1.4. G-patch proteins
    • 1.5. Aims
  • 2. MATERIALS AND METHODS
    • 2.1. Materials
    • 2.2. Methods
  • 3. RESULTS
    • 3.1. Identification of Cmg1-interacting helicases
    • 3.2. Analysis of the Cmg1 - Prp43 interactionin vitro
    • 3.3. Identification of Cmg1 and Prp43interacting domains
    • 3.4. Effect of Cmg1 on the RNA binding affinityof Prp43
    • 3.5. Prp43 ATPase activity in the presence ofCmg1
    • 3.6. Cmg1 cellular localisation
    • 3.7. Submitochondrial localisation of Cmg1
    • 3.8. Analysis of mitochondrial metabolism uponcmg1 deletion or overexpression
    • 3.9. Cell growth and survival in cmg1 deletion and overexpression strains
    • 3.10. Relocalisation of Cmg1 and Prp43 underapoptotic conditions
    • 3.11. Mitochondrial binding partners of Prp43 inapoptosis
    • 3.12. Prp43 localisation in the complex IIIdeficient strain
    • 3.13. Localisation of Prp43 upon G-patch proteinoverexpression
    • 3.14. G-patch proteins compete for Prp43binding
    • 3.15. Overexpression of G-patch proteins affectsprecursor ribosomal RNA processing
  • 4. DISCUSSION
    • 4.1. Characterisation of the orphan G-patchprotein Cmg1
    • 4.2. Prp43 at mitochondria in apoptosis
    • 4.3. Recruitment of helicases to their target sites
    • 4.4. Regulation of RNA helicases by cofactorcompetition
    • 4.5. Conclusions
  • 5. REFERENCES
  • PUBLICATION
  • ACKNOWLEDGMENTS
  • CURRICULUM VITAE

Publikationstyp: Hochschulschrift

Sparte: Universitätsverlag

Sprache: Englisch

ISBN: 978-3-86395-237-2 (Print)

URN: urn:nbn:de:gbv:7-isbn-978-3-86395-237-2-3

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